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Safety Data Sheet (SDS) FITC Anti-mouse I-Ak Aalphak Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse I-Ak (Aαk) Antibody
1150030 500 µg $245.00       
Clone: 11-5.2
Isotype: Mouse (BALB/c) IgG2b, κ
Reactivity: Mouse k haplotype, Cross-reacts with H-2r haplotypes. Does not cross react with other haplotypes. (e.g., b, d, f, p, q, s).
Immunogen: CKB mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking of MHC class II - restricted CD4+ T cell responses2,3, induction of B cell differentiation4, and immunohistochemical staining5 of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Oi VT, et al. 1978. Curr. Top. Microbiol. Immunol. 81:115. (IP)
2. Formby B, et al. 1990. P. Natl. Acad. Sci. USA 87:2438.
3. Kappler JW, et al. 1981. J. Exp. Med. 153:1198.
4. Bishop GA, et al. 1989. P. Natl. Acad. Sci. USA 86:5933.
5. Ardehali A, et al. 1995. Circulation 92:450. (IHC)
6. Turnquist HR, et al. 2007. J. Immunol. 178:7018.

C3H mouse splenocytes stained with

C3H mouse splenocytes stained with 11-5.2 FITC



Description:

The 11-5.2 antibody reacts with the α chain of the I-Ak MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 11-5.2 antibody cross-reacts with cells from mice bearing the H-2r haplotype. Reactivity with other H-2 haplotypes (e.g., b, d, f, p, q, s) has not been observed. The 11-5.2 antibody has been reported to block antigen presentation and induce B cell differentiation.

Other Names: MHC class II
Structure: α chain of MHC class II
Distribution: B cell and activated T cells, APCs of H-2b mice
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD4
Antigen References:

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.