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Safety Data Sheet (SDS) PE Anti-mouse/rat/human MCP-1 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse/rat/human MCP-1 Antibody
3129515 25 µg $100.00       
3129520 100 µg $235.00       
Clone: 2H5
Isotype: Armenian Hamster IgG
Reactivity: Mouse, Rat, Human, Cross-Reactivity: Rhesus
Immunogen: CHO-expressed, recombinant mouse MCP-1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Application Notes:

ELISA or ELISPOT Capture1: The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody as the detecting antibody for the detection of human MCP-1.

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining by flow cytometry, the suggested use of this product is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Luo, Y., et al. 1994. J. Immunol. 153:3708.
2. Zhang, Y., et al. 2002. J. Immunol. 168:3088.
3. Luo, Y., et al. 1999 J. Immunol. 163:3985.
4. Morrison, B. E., et al. 2003 J. Clin. Invest. 112:1862.
5. Hancock, W. W., et al. 1997 Transplantation 64:696.
6. Yu, R., et al. 2006 Obesity 14:1353.
7. Taal MW, et al. 2001. Am. J. Physiol. Renal. Physiol. 280:F343.
8. Wan W, et al. 2015. Cardiovasc Res. 106:478. PubMed

LPS-stimulated human peripheral blood monocytes

LPS-stimulated human peripheral blood monocytes were surface stained with CD14 FITC and then intracellular stained with 2H5 PE



Description:

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 2H5 antibody reacts with mouse, rat, and human MCP-1. The 2H5 antibody can neutralize the bioactivity of natural or recombinant MCP-1.

Other Names: Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2
Structure: CC chemokine; dimer; 13.8 kD (Mammalian)
Regulation: Upregulated by IL-1, TNF-α, PDGF, TGF-β
Cellular Sources: Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells
Cellular Targets: Monocytes, basophils, T cells, NK cells, immature dendritic cells
Receptors: CCR2; CCR5; CCR10; D6; possibly L-CCR
Bioactivity/Activities: Chemoattractant; regulates adhesion molecule expression, cytokine production in monocytes; proliferation/activation of CC-chemokine activated killer cells
Antigen References:

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bischoff, S., et al. 1992. J. Exp. Med. 175:1271.
3. Charo, I., et al. 1994. P. Natl. Acad. Sci. USA 91:2752.
4. Jiang, Y., et al. 1992. J. Immunol. 148:2423.