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Safety Data Sheet (SDS) PE Anti-human IFN-gamma Antibody     Product Data Sheet (PDF)    
PE Anti-human IFN-γ Antibody
3112540 25 tests $95.00       
3112550 50 µg $130.00       
3112545 100 tests $205.00       
Clone: 4S.B3
Isotype: Mouse IgG1, κ
Reactivity: Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus
Immunogen: Partially purified, native human IFN-γ
Formulation: µg format: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Test format: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w/v) BSA (USA origin).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: µg sizes: 0.2 mg/ml
test sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Application Notes:

ELISA or ELISPOT Detection5: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody or purified MD-1 antibody as the capture antibody.
Flow Cytometry3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.
Additional reported applications (for the relevant formats) include: neutralization1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Meager A, et al. 1984. J. Interferon Res. 4:619. (Neut)
2. Meager A, 1987. Lymphokines and Interferons:A Practical Approach. IRL Press Ltd, Oxford, p. 105. (Neut)
3. Sester M, et al. 2002. J. Virol. 76:3748. (ICFC)
4. Infante-Duarte C, et al. 2000 J. Immunol. 165:6107. (ICFC)
5. Goodier M, et al. 2000. J. Immunol. 165:139. (ELISA)
6. Chen H, et al. 2005. J. Immunol. 175:591. (ICFC)
7. Smeltz RB, 2007. J. Immunol. 178:4786. (ICFC)
8. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (ICFC)
10. Longhi MS, et al. 2014. PLoS One. 9:87956. PubMed
11. Chandran PA, et al. 2014. J Leukoc Biol. 96:633. PubMed

PMA/Ionomycin-stimulated human PBMCs were stained

PMA/Ionomycin-stimulated human PBMCs were stained with CD3 PE/Cy5 and 4S.B3 PE



Description:

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Other Names: Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Structure: Cytokine; dimer; 20-25 kD (Mammalian)
Regulation: Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Cellular Sources: CD8+ and CD4+ T cells, NK cells
Cellular Targets: T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Bioactivity/Activities: Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Antigen References:

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.