The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of costimulation of T cells1-3, and immunohistochemical staining of acetone-fixed frozen sections3.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Maeda, K., et al. 1997. Int. Immunol. 9:993. (IP, Block) 2. Bluestone, J.A. 1995. Immunity 2:555. (IP, Block) 3. Damoiseaux, J.G., et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC)
LPS-stimulated (3 days) LOU rat splenocytes stained with biotinylated 24F, followed by SAV-PE
CD86 is an 80 kD member of the immunoglobulin superfamily also known as B70 or B7-2. It is expressed on antigen presenting cells and activated T and B cells. CD86 is a ligand for CD28 and CD152 (CTLA-4). It is one of the accessory molecules that plays an important role in T cell-APC cell costimulatory interactions. The 24F antibody can block the co-stimulatory activity of CD86.
Ig superfamily, 80 kD
Antigen presenting cells, activated B cells and T cells
T and B cell costimulation
CD28, CD152 (CTLA-4)
1. Barclay, A.N. et al. 1997. The Leukocyte Antigen FactsBook. Academic Press. 2. Hathcock, K.S., et al. 1993. Science 262:905. 3. Freeman, G.J., et al. 1993. Science 262:907. 4. Carreno, B.M., et al. 2002. Ann. Rev. Immunol. 20:29.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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