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Safety Data Sheet (SDS) Purified Anti-rat CD80 Antibody     Product Data Sheet (PDF)    
Purified Anti-rat CD80 Antibody
1601010 500 µg $215.00       
Clone: 3H5
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: HTLV-1-transformed Lewis-S1 rat T cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IP, IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of ligand binding1-3, and immunohistochemical staining of acetone-fixed frozen sections3.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Maeda, K., et al. 1997. Int. Immunol. 9:993. (IP, Block)
2. Bluestone, J.A., 1995. Immunity 2:555. (IP, Block)
3. Damoiseaux, J.G., et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC)
4. Ho, CH., et al. 2007. J. Virol. 81:4837. PubMed
5. Ho, C., et al. 2009. Carcinogenesis 30:1443. PubMed

LPS-stimulated LOU rat splenocytes (3

LPS-stimulated LOU rat splenocytes (3 days) were stained with CD80 (clone 3H5) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).



Description:

CD80 is a 60 kD member of the immunoglobulin superfamily also known as B7-1, B7, and BB1. It is expressed on antigen presenting cells and activated T and B cells, and is a ligand for CD28 and CD152 (CTLA-4). CD80/CD28 interactions are important for T and B cell co-stimulation, while CD80/CD152 lead to the inhibition of T cell responses. The 3H5 antibody can block the co-stimulatory activity of CD80.

Other Names: B7-1, B7, BB1, Ly-53
Structure: Ig superfamily, 60 kD
Distribution: Antigen presenting cells, activated B cells and T cells
Function: T and B cell costimulation
Ligand Receptor: CD28, CD152 (CTLA-4)
Antigen References:

1. Barclay, A.N. et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Linsley, P.S., et al. 1991. J. Exp. Med. 174:561.
3. Salomon, B., et al. 2001. Ann. Rev. Immunol. 19:225.