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Safety Data Sheet (SDS) PE Anti-rat CD106 Antibody     Product Data Sheet (PDF)    
PE Anti-rat CD106 Antibody
1602015 50 µg $100.00       
Clone: MR106
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: Rat VCAM-1 transfected L5178Y cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2,3.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Bevilacqua, M.P. 1993. Annu. Rev. Immunol. 11:767. (IHC)
2. Wingerd, K.L., et al. 2002. J. Neuroscience 22:10772. (IHC)
3. Ogawa, T., et al. 2004. Am J. Physiol. Gastrointest. Liver Physiol. 286:G702. (IHC)

Lou rat bone marrow cells

Lou rat bone marrow cells stained with MR106 PE



Description:

CD106 is a 110 kD transmembrane protein also known as VCAM-1 and INCAM-110. It is an immunoglobulin superfamily member and is expressed on bone marrow stromal cells, myeloid cells, splenic dendritic cells and endothelial cells (activated by cytokines). CD106 is a counter-receptor for VLA-4 (α4β1 integrin) and LPAM-1 (α4β7 integrin). The interactions of CD106 and VLA-4 or LPAM-1 play an important role in binding of leukocytes to activated endothelial cells and leukocyte extravasation at inflammatory sites.

Other Names: VCAM-1, INCAM-110
Structure: Ig superfamily, 110 kD
Distribution: Bone marrow stromal cells, myeloid cells, splenic dendritic cells, induced on endothelial cells
Function: Adhesion, leukocyte extravasation at sites of inflammation
Ligand Receptor: VLA-4 (α41 integrin) and LPAM-1 (α47 integrin)
Antigen References:

1. Barclay, A.N., et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Kinashi, T., et al. 1995. J. Leuk. Biol. 57:168.
3. Bevilacquea, M.P., 1993. Ann. Rev. Immunol. 11:767.
4. Koni, P.A., et al. 2001. J. Exp. Med. 193:741.