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Safety Data Sheet (SDS) Purified Anti-rat CD106 Antibody     Product Data Sheet (PDF)    
Purified Anti-rat CD106 Antibody
1602005 50 µg $70.00       
1602010 500 µg $215.00       
Clone: MR106
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: Rat VCAM-1 transfected L5178Y cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2,3.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Bevilacqua, M.P. 1993. Annu. Rev. Immunol. 11:767. (IHC)
2. Wingerd, K.L., et al. 2002. J. Neuroscience 22:10772. (IHC)
3. Ogawa, T., et al. 2004. Am J. Physiol. Gastrointest. Liver Physiol. 286:G702. (IHC)

Lou rat bone marrow cells

Lou rat bone marrow cells stained with MR106 PE



Description:

CD106 is a 110 kD transmembrane protein also known as VCAM-1 and INCAM-110. It is an immunoglobulin superfamily member and is expressed on bone marrow stromal cells, myeloid cells, splenic dendritic cells and endothelial cells (activated by cytokines). CD106 is a counter-receptor for VLA-4 (α4β1 integrin) and LPAM-1 (α4β7 integrin). The interactions of CD106 and VLA-4 or LPAM-1 play an important role in binding of leukocytes to activated endothelial cells and leukocyte extravasation at inflammatory sites.

Other Names: VCAM-1, INCAM-110
Structure: Ig superfamily, 110 kD
Distribution: Bone marrow stromal cells, myeloid cells, splenic dendritic cells, induced on endothelial cells
Function: Adhesion, leukocyte extravasation at sites of inflammation
Ligand Receptor: VLA-4 (α41 integrin) and LPAM-1 (α47 integrin)
Antigen References:

1. Barclay, A.N., et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Kinashi, T., et al. 1995. J. Leuk. Biol. 57:168.
3. Bevilacquea, M.P., 1993. Ann. Rev. Immunol. 11:767.
4. Koni, P.A., et al. 2001. J. Exp. Med. 193:741.