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Safety Data Sheet (SDS) FITC Anti-rat CD49d Antibody     Product Data Sheet (PDF)    
FITC Anti-rat CD49d Antibody
1600515 50 µg $90.00       
Clone: MRα4-1
Isotype: Mouse IgG2a, κ
Reactivity: Rat
Immunogen: RBL-2H3 rat basophilic leukemia cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications include: immunoprecipitation1, in vitro activation, in vitro inhibition1 of binding of VCAM-1 and mast cells to fibronectin , and in vivo inhibition of VLA-4 functions1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Yasuda M, et al. 1995. Int. Immunol. 7:251. (IP, Block)

Lou rat blood lymphocytes stained

Lou rat blood lymphocytes stained with MRα4-1 FITC



Description:

CD49d is a 150 kD glycoprotein, also known as integrin α4 or VLA-4 α chain. It is expressed as a heterodimer with either of two β chains, β1 or β7. The α4β1 integrin (also known as VLA-4, CD49d/CD29) is expressed on peripheral T and B lymphocytes, thymocytes, and monocytes, while the α4β7 integrin (also known as LPAM-1) is expressed on peripheral lymphocytes. The α4β1 and α4β7 integrins mediate cell-cell and cell-matrix interactions by interacting with the ligands CD106 (VCAM-1), MAdCAM-1, and fibronectin. Soluble MRα4-1 antibody can partially inhibit in vitro binding of VCAM-1 and mast cells to fibronectin and inhibit mast cell degranulation. Immobilized MRα4-1 antibody can enhance mast cell degranulation in vitro.

Other Names: Integrin α4 chain, α4 integrin, VLA-4 α chain, ITGA4
Structure: Integrin α chain, 150 kD
Distribution: α41- peripheral T cells and B cell lymphocytes, thymocytes, and monocytes; α47- peripheral lymphocytes
Function: Adhesion
Ligand Receptor: CD106 (VCAM-1), MAdCAM-1, fibronectin
Antigen References:

1. Yasuda, M., et al. 1995. Int. Immunol. 7:251.
2. Springer, T.A. 1994. Cell 76:301.
3. Issekutz, T. B., et al. 1991. J. Immunol. 147:4178.
4. Issekutz, T. B., et al. 1991. J. Immunol. 147:109.
5. Yang, H., et al. 1995. Transplantation 60:71.
6. Palecanda, A., et al. 1997. J. Immunol. 158:2904.