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Safety Data Sheet (SDS) Purified Anti-mouse CD28 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD28 Antibody
1110505 50 µg $50.00       
1110510 500 µg $190.00       
Clone: 37.51
Isotype: Syrian Hamster IgG
Reactivity: Mouse
Immunogen: C57BL/6 mouse T-cell lymphoma EL-4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The CD28 antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IP, IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity3,4, in vitro induction of thymocyte differentiation2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim)
2. Cibotti R, et al. 1997. Immunity 6:245. (Costim)
3. Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block)
4. Nishio M, et al. 1996. J. Immunol. 157:4347. (Block)
5. Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim)
6. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
7. Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim)
8. Wang W, et al. 2007. J. Immunol. 178:4885. (Costim)
9. Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim)
10. Perchonock CE, et al. 2007. J. Immunol. 179:1768.
11. Barbi J, et al. 2007. Blood 110:2215.
12. Milpied P, et al. 2011. Blood 118:2993. PubMed
13. Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed
14. Crispin JC, et al. 2012. J. Immunol. 188:3567. PubMed
15. Li CR, et al. 2014. J Immunol. 192:1425. PubMed
16. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed

C57BL/6 splenocytes stained with 37.51

C57BL/6 splenocytes stained with 37.51 PE and CD3 FITC



Description:

CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.

Other Names: Tp44, T44
Structure: Ig superfamily, 44 kD
Distribution: Thymocytes, CD4+, CD8+ peripheral T cells, NK cells
Function: Costimulates T and NK cells
Ligand Receptor: CD80 (B7-1), CD86 (B7-2)
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380.