The antibody was purified by affinity chromatography.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested IP, IHC - Reported in the literature
Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen sections, in vitro blocking of ligand binding1, and immunoprecipitation1.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Xu H, et al. 1996. J. Immunol. 156:4909. 2. Gerwin N, et al. 1999. Immunity 10:9.
C57BL/6 mouse splenocytes stained with purified 3C4,followed by anti-rat IgG FITC
CD102 is a 55 kD immunoglobulin superfamily member, also known as ICAM-2. CD102 is a heavily glycosylated cell surface ligand for LFA-1 (CD11a/CD18). CD102 is constitutively expressed on vascular endothelial cells, some lymphocytes, monocytes, and platelets. CD102 is thought to be involved in lymphocyte recirculation and T cell activation. The 3C4(MIC2/4) antibody can block interactions between ICAM-2 and LFA-1.
Ig superfamily, 55 kD
Vascular endothelial cells, some resting lymphocytes, monocytes, platelets
Lymphocyte recirculation, costimulation
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Casasnovas JM, et al. 1999. P. Natl. Acad. Sci. USA 96:3017. 3. Diamond MS, et al. 1990. J. Cell Biol. 111:3129.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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