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Safety Data Sheet (SDS) Purified Anti-mouse CD102 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD102 Antibody
1128005 50 µg $70.00       
1128010 500 µg $210.00       
Clone: 3C4 (MIC2/4)
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: COS cells transfected with mouse ICAM-2 cDNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IP, IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen sections, in vitro blocking of ligand binding1, and immunoprecipitation1.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References: 1. Xu H, et al. 1996. J. Immunol. 156:4909.
2. Gerwin N, et al. 1999. Immunity 10:9.
C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with purified 3C4,followed by anti-rat IgG FITC



Description: CD102 is a 55 kD immunoglobulin superfamily member, also known as ICAM-2. CD102 is a heavily glycosylated cell surface ligand for LFA-1 (CD11a/CD18). CD102 is constitutively expressed on vascular endothelial cells, some lymphocytes, monocytes, and platelets. CD102 is thought to be involved in lymphocyte recirculation and T cell activation. The 3C4(MIC2/4) antibody can block interactions between ICAM-2 and LFA-1.
Other Names: ICAM-2
Structure: Ig superfamily, 55 kD
Distribution: Vascular endothelial cells, some resting lymphocytes, monocytes, platelets
Function: Lymphocyte recirculation, costimulation
Ligand Receptor: LFA-1, Mac-1
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Casasnovas JM, et al. 1999. P. Natl. Acad. Sci. USA 96:3017.
3. Diamond MS, et al. 1990. J. Cell Biol. 111:3129.