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Safety Data Sheet (SDS) Purified Anti-mouse CD279 PD-1 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD279 (PD-1) Antibody
1170505 50 µg $75.00       
1170510 500 µg $225.00       
Clone: RMP1-14
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse PD-1 transfected BHK cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze."
Application:

FC - Quality tested
IHC , WB - Reported in the literature

Application Notes:

Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of acetone-fixed frozen sections1, Western blotting2,6, inhibition of macrophages (but not B cells and dendritic cells) costimulated T cell proliferation3,5, and blocking the binding of PD-1 to B7-H1 or B7-DC3,4,8.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Kanai, T., et al. 2003. J. Immunol. 171:4156. (FC, IHC)
2. Personal Communication (WB)
3. Yamazaki, T., et al. 2005. J. Immunol. 175:1586. (Costim)
4. Matsumoto, K., et al. 2004. J. Immunol. 172:2530. (Block)
5. Terrazas, L. I., et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
6. Meng, Q., et al. 2006. Invest Opthalmol Vis Sci. 47:444. (FC, WB) PubMed
7. Tsang JY, et al. 2011. Int Immunopharmacol. 11:604. PubMed
8. Takamura S, et al. 2010. J. Immunol. 184:4696. PubMed (Block)

Con A-stimulated C57BL/6 splenocytes stained

Con A-stimulated C57BL/6 splenocytes stained with purified RMP1-14, followed by biotinylated anti-rat IgG and Sav-PE



Description:

CD279 is a 50-55 kD immunoglobulin superfamily member, also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production costimulated by macrophages (but not by dendritic cells and B cells).

Other Names: Programmed Death-1, CD279, PD 1
Structure: Ig superfamily, 50-55 kD
Distribution: Subset of double negative thymocytes, activated T and B cells
Function: Lymphocyte clonal selection, peripheral tolerance
Ligand Receptor: PD-L1 (B7-H1), PD-L2
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook, Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.