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Safety Data Sheet (SDS) Biotin Anti-mouse CD210 IL-10 R Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse CD210 (IL-10 R) Antibody
1163520 500 µg $235.00       
Clone: 1B1.3a
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: Soluble mIL-10R-FLAG-His
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of ligand binding1-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody or biotinylated anti-rat IgG second step, followed by SAv-PE ).

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. O'Farrell A, et al. 1998. EMBO J. 17:1006. (Block)
2. Wang W, et al. 2004. FASEB J. 18:1043. (Block)
3. Vieira PL, et al. 2004. J. Immunol. 172:5986. (Block)
4. Iwashiro M, et al. 2001. P. Natl. Acad. Sci. USA 98:9226. (Block)
5. Vicari AP, et al. 2002. J. Exp. Med. 196:541. (Block)
6. Girard-Madoux MJ, et al. 2012. J Allergy Clin Immunol. 129:143. PubMed.

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with biotinylated 1B1.3a, followed by Sav-PE



Description:

CD210 is a 90-110 kD IL-10 receptor. It is a class II cytokine receptor expressed on thymocytes, T cells, B cells, NK cells, monocytes and macrophages. Ligand binding of CD210 induces Jak1 and Tyk, resulting in STAT1 and STAT3 activation. IL-10 receptor stimulation results in the inhibition of cytokine production and the costimulation of B cell proliferation and differentiation. The only known ligand for this receptor is IL-10.

Other Names: IL-10R, Interleukin-10 Receptor
Structure: Class II cytokine receptor family, 90-110 kD
Distribution: Thymocytes, T and B cells, NK cells, monocytes, macrophages
Function: Inhibits cytokine production, costimulates B cell proliferation and differentiation
Ligand Receptor: IL-10
Antigen References:

1. Ho A, et al. 1993. P. Natl. Acad. Sci. USA 90:11267.
2. Tan JC, et al. 1993. J. Biol. Chem. 268:21053.
3. Niemand C, et al. 2003. J. Immunol. 170:3263.
4. Corinti S, et al. 2001. J. Immunol. 166:4312.