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Safety Data Sheet (SDS) Purified Anti-mouse CD119 IFN-gamma R Alpha Chain Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD119 (IFN-γ R α Chain) Antibody
1164010 500 µg $205.00       
Clone: 2E2
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Soluble recombinant mouse IFN-γR α chain protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IP, WB - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation1. The 2E2 antibody does not block the binding of IFN-γ to IFN-γ receptor. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody or biotinylated anti-Armenian hamster IgG second step, followed by SAv-PE ).

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Bach EA, et al. 1995. Science 270:1215.

C57BL/6 splenocytes stained with purified

C57BL/6 splenocytes stained with purified 2E2, then detected with biotinylated anti-Armenian hamster IgG followed by Sav-PE



Description:

CDw119 is a 90 kD immunoglobulin superfamily member, also known as IFN-γRα chain. It is a class II cytokine receptor family member that serves as a IFN-γ-binding chain associated with the IFN-γ β chain also known as AF-1. In addition to ligand binding, CDw119 participates in ligand trafficking. CDw119 is expressed on T and B cells, NK cells, fibroblasts, endothelial, and epithelial cells. Binding of IFN-γ induces receptor dimerization, internalization, Jak1 and Jak2 protein kinase activation and, ultimately, STAT1 activation. IFN-γ initiates and regulates a variety of immune responses.

Other Names: IFN-γRα , IFN-γ Receptor α chain, CDw119
Structure: Ig superfamily, class II cytokine receptor family, associates with IFN-γ AF-1, 90 kD
Distribution: T cells and B cells, NK cells, myeloid cells, fibroblasts, endothelial and epithelial cells
Function: Initiates and regulates immune response
Ligand Receptor: IFN-γ
Antigen References:

1. Basu M, et al. 1988. P. Natl. Acad. Sci. USA 85:6282.
2. Codias EK, et al. 1990. J. Immunol. 144:2197.