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Safety Data Sheet (SDS) PE Anti-rat IFN-gamma Antibody     Product Data Sheet (PDF)    
PE Anti-rat IFN-γ Antibody
3139030 100 tests $195.00       
Clone: DB-1
Isotype: Mouse IgG1, κ
Reactivity: Mouse, Rat
Immunogen: Recombinant rat IFN-γ
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Application Notes: ELISA Capture1 or ELISPOT Capture2: The purified DB-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated poly5109 antibody as the detecting antibody and recombinant IFN-γ as the standard.
Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References: 1. Van der Meide P, et al. 1989. Lymphokine Res. 8:439.
2. Nennesmo I, et al. 1989. Brain Res. 504:306.
3. Rayner D, et al. 1987. Scand. J. Immunol. 25:621.
4. Hartung H, et al. 1990. Ann Neurol. 27:247.
5. Bernard I, et al. 1998. Eur. Cytokine Net. 9:613.
PMA/lonomycin stimulated Lou rat splenocytes

PMA/lonomycin stimulated Lou rat splenocytes were stained with DB-1 PE.



Description: Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The DB-1 antibody reacts with rat and mouse interferon-gamma (IFN-γ). The DB-1 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The DB-1 antibody has been well characterized for ELISPOT, ELISA, intracellular staining, Western blotting, IHC, and neutralization (in vitro and in vivo).
Other Names: Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Structure: Cytokine; dimer; 40-80 kD (Mammalian)
Regulation: Upregulated by IL-2, bFGF, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Cellular Sources: CD8+ and CD4+ T cells, NK cells
Cellular Targets: T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Bioactivity/Activities: Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu .Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.