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Safety Data Sheet (SDS) Purified Anti-rat IFN-gamma Antibody     Product Data Sheet (PDF)    
Purified Anti-rat IFN-γ Antibody
3139005 50 µg $90.00       
3139010 500 µg $225.00       
Clone: DB-1
Isotype: Mouse IgG1, κ
Reactivity: Mouse, Rat
Immunogen: Recombinant rat IFN-γ
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

ELISA Capture - Quality tested
ICFC, IHC, WB - Reported in the literature

Application Notes:

ELISA Capture1 or ELISPOT Capture2: The purified DB-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated poly5109 antibody as the detecting antibody and recombinant IFN-γ as the standard.

Recommended Usage:

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, the suggested use of this reagent is 1-4 ug/ml. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. The purified DB-1 has been tested by blocking fluorochrome conjugated DB-1 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody (≤ 5.0 ug/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Van der Meide P, et al. 1989. Lymphokine Res. 8:439.
2. Nennesmo I, et al. 1989. Brain Res. 504:306.
3. Rayner D, et al. 1987. Scand. J. Immunol. 25:621.
4. Hartung H, et al. 1990. Ann Neurol. 27:247.
5. Bernard I, et al. 1998. Eur. Cytokine Net. 9:613.





Description:

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The DB-1 antibody reacts with rat and mouse interferon-gamma (IFN-γ). The DB-1 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The DB-1 antibody has been well characterized for ELISPOT, ELISA, intracellular staining, Western blotting, IHC, and neutralization (in vitro and in vivo).

Other Names: Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Structure: Cytokine; dimer; 40-80 kD (Mammalian)
Regulation: Upregulated by IL-2, bFGF, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Cellular Sources: CD8+ and CD4+ T cells, NK cells
Cellular Targets: T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Bioactivity/Activities: Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Antigen References:

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu .Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.