The antibody was purified by affinity chromatography.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Hasendrug K, et al. 1987. Immunogenetics 25:136. 2. Kafrouni MI, et al. 2001. J. Immunol. 167:1566. 3. Nanni P, et al. 2001. J. Exp. Med. 194:1195.
FVB mouse splenocytes stained with purified KH114, followed by anti-mouse IgG2a FITC
The KH114 antibody reacts with the H-2Kq MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kq haplotype. H-2Kq is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The KH114 antibody has not been reported to cross-react with other haplotypes.
MHC class I
MHC class I
Nucleated cells of the H-2Kq haplotype
1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. 3. York I, et al. 1996. Ann. Rev. Immunol. 14:369. 4. Hasendrug K, et al. 1987. Immunogenetics 25:136.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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