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Safety Data Sheet (SDS) Purified Anti-mouse H-2Kq Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse H-2Kq Antibody
1175505 50 µg $75.00       
Clone: KH114
Isotype: Mouse (BALB/c) IgG2a, κ
Reactivity: Mouse q haplotype, Does not cross-react with other haplotypes (e.g. a, b, d, f, k, n, p, r, s, u, v)
Immunogen: B10.Q mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References: 1. Hasendrug K, et al. 1987. Immunogenetics 25:136.
2. Kafrouni MI, et al. 2001. J. Immunol. 167:1566.
3. Nanni P, et al. 2001. J. Exp. Med. 194:1195.
FVB mouse splenocytes stained with

FVB mouse splenocytes stained with purified KH114, followed by anti-mouse IgG2a FITC



Description: The KH114 antibody reacts with the H-2Kq MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kq haplotype. H-2Kq is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The KH114 antibody has not been reported to cross-react with other haplotypes.
Other Names: MHC class I
Structure: MHC class I
Distribution: Nucleated cells of the H-2Kq haplotype
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD8
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
4. Hasendrug K, et al. 1987. Immunogenetics 25:136.