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Safety Data Sheet (SDS) Purified Anti-Phosphotyrosine Antibody     Product Data Sheet (PDF)    
Purified Anti-Phosphotyrosine Antibody
2146505 25 µg $50.00       
2146510 100 µg $95.00       
Clone: PY20
Isotype: Mouse IgG2b, κ
Reactivity: All Species
Immunogen: KLH-conjugated phosphotyrosine
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol. Final antibody concentration is 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored at -20°C.
Application:

WB - Quality tested
ICFC, IF, IP- Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, immunofluorescence microscopy3.

Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. Suggested working dilution(s): Use 1-2 µg per ml antibody dilution buffer per mini-gel. Do not use dilution or blocking buffers containing milk as they may interfere with antibody binding to proteins of interest. Dilution and blocking buffers containing 4% bovine serum albumin are recommended for use with this antibody. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Vuori K, et al. 1995. J. Biol. Chem. 270:22259. (IP, WB)
2. Glenney J, et al. 1988. J. Immunol. Meth. 109:277. (IP, WB)
3. Prahalad P, et al. 2004. Am J Physiol Cell Physiol 286:C693. (IF)
4. Zentillin L, et al. 2009. FASEB J. 24:1467. PubMed
5. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
6. Cespedes PF, et al. 2014. PNAS. 111:3214. PubMed

Hela cell extract was resolved

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-phosphotyrosine antibody (clone PY-20).
Lane 1, serum-starved Hela cells;
Lane 2, serum-starved Hela cells following serum addition for 4 hrs.
Lane 2 shows an upregulation of tyrosine phosphorylated proteins after serum addition. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.



Description:

Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.

Other Names:
Distribution: Phospho-Specific
Function: Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation