Sony Biotechnology Inc. Sony Biotechnology Inc.cart
About Us Products What's New Library News Calendar Contact Us Place Order
Safety Data Sheet (SDS) PE Anti-mouse CD123 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD123 Antibody
1130025 25 µg $75.00       
Clone: 5B11
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: CTLL cells transfected with the mouse IL-3Rα chain
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody or biotinylated anti-rat IgG second step , followed by SAv-PE ). To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 , is recommended prior to immunofluorescent staining.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Ichihara, et al. 1995. EMBO J. 14:939. (FC)
2. Mueller DL, et al. 1994. J. Immunol. 153:3014. (FC)
3. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC)

C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells stained with 5B11 PE and CD11b FITC



Description:

CD123 is a 70 kD α chain subunit of the IL-3 receptor (IL-3R α). It is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL-3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either the common β chain (CDw131) or the IL-3R β chain (AIC2A) to form high-affinity heterodimeric IL-3 receptors. CDw131 can complex with the α subunits of the mouse IL-3R, IL-5R and GM-CSFR to form high-affinity receptors, while the IL-3 R β subunit is specific for IL-3 but binds with low affinity. IL-3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. The 5B11 antibody does not block binding of IL-3 to the high affinity IL-3 receptor.

Other Names: IL-3 Receptor α chain, IL-3Rα
Structure: Ig superfamily, associates with CDw131 (common β chain) or IL-3 receptor β chain, 70 kD
Distribution: Hematopoietic progenitors, basophils, mast cells, megakaryocytes
Function: Proliferation, differentiation of hematopoietic cells
Ligand Receptor: IL-3
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Miyajima A, et al. 1993. Blood 82:1960.
3. Itoh N, et al. 1990. Science 247:324.