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Safety Data Sheet (SDS) PE Anti-mouse H-2K B/H-2D B Antibody     Product Data Sheet (PDF)    
PE Anti-mouse H-2K b/H-2D b Antibody
1173035 50 µg $100.00       
1173040 200 µg $265.00       
Clone: 28-8-6
Isotype: Mouse (C3H) IgG2a, κ
Reactivity: Mouse b haplotype, Cross-reacts with H-2Dd MHC class I alloantigen. Does not react with other haplotypes (e.g., f, k, p, q, r, s)
Immunogen: C3H.SW mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Ozato K, et al. 1981. J. Immunol. 126:317. (Cyt)
2. Pappo J, et al. 1999. Infect. Immun. 67:337. (IHC)
3. Bui JD, et al.2006. J. Immunol. 176:905. (FC) PubMed.
4. Shao H, et al. 2005. J. Immunol. 175:1851. (FC)
5. Coulouarn, C., et al. 2011. Carcinogensis. 32:1434. PubMed.

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with 28-8-6 PE



Description:

The 28-8-6 antibody reacts with the H-2Kb and H-2Db MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kb/H-2Db haplotype. H-2Kb/H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-8-6 antibody cross-reacts with H-2Dd MHC class I alloantigen, but does not react with alloantigens of f, k, p, q, r, s haplotypes.

Other Names: MHC class I
Structure: MHC class I
Distribution: Nucleated cells of the H-2Kb/H-2Db haplotype
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD8
Antigen References:

1. Ozato K, et al. 1981. J. Immunol. 126:317.
2. Allen H, et al. 1986. P. Natl. Acad. Sci. USA 83:7447.
3. Evans GA, et al. 1992. Nature 300:755.