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Safety Data Sheet (SDS) PE Anti-mouse H-2Ld/H-2Db Antibody     Product Data Sheet (PDF)    
PE Anti-mouse H-2Ld/H-2Db Antibody
1172535 50 µg $95.00       
Clone: 28-14-8
Isotype: Mouse (C3H/HeJ) IgG2a, κ
Reactivity: Mouse b and d haplotype, Cross-reacts with H-2Ld. Does not react with other haplotypes (e.g., k, f, p, r, s)
Immunogen: C3H.SW mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation3, blocking of cell lysis, and immunohistochemical staining of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Ozato K, et al. 1980. J. Immunol. 125:2473.
2. Kafrouni MI, et al. 2001. J. Immunol. 167:1566.
3. Marguet D, et al. 1999. Immunity 11:231.
4. Bashuda H, et al. 1997. Transplantation 63:113.
5. Greeneltch KM, et al. 2007. Cancer Res.67:10406. PubMed
6. Milstein O, et al. 2011. Blood 117:1042.

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with 28-14-8 PE



Description:

The 28-14-8 antibody reacts with the H-2Db MHC class I alloantigen expressed on nucleated cells from mice of the H-2Db haplotype. H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-14-8 antibody has been reported to block antigen-specific lysis of target cells by cytotoxic T lymphocytes. The 28-14-8 antibody cross-reacts with the H-2Ld haplotype. Clone 28-14-8 recognizes the α3 domain of H-2Db.

Other Names: MHC class I
Structure: MHC class I
Distribution: Nucleated cells of the H-2Ld/H-2Db haplotype
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD8
Antigen References:

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York IA, et al. 1996. Ann. Rev. Immunol. 14:369.