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Safety Data Sheet (SDS) Purified Anti-mouse CD8a Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD8a Antibody
1104005 50 µg $75.00       
1104010 500 µg $210.00       
Clone: 5H10-1
Isotype: Rat (SD) IgG2b, λ
Reactivity: Mouse
Immunogen: Concanavalin A-stimulated BALB/c splenic T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IF - Validated
IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: partial inhibition of T-cell responses to IL-2 and cytotoxic induction of splenic T cells1, immunohistochemistry of acetone-fixed frozen sections2, and immunofluorescence. The 5H10-1 antibody competes with 53-6.7 antibody for binding to thymocytes1.

Clone 5H10-1 is not recommended for immunohistochemistry of formalin-fixed paraffin sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557.
2. Frei K, et al. 1997. J. Exp. Med. 185:2177.

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with purified 5H10-1, followed by anti-rat IgGs-FITC

Mouse OT-I primary CTL cells

Mouse OT-I primary CTL cells were stained with purified anti-mouse CD8a primary antibody at 1:50 dilution in PBS/1%NGS incubated O/N at 4°C, detected with anti-rat IgG2b FITC at 1:2000 dilution for 1hr at room temperature (green), plus DAPI staining for nuclei (blue). Imaged with a Zeiss 200M deconvolution microscope (deconvolution applied). Negative control was anti-rat IgG2b isotype control plus secondary antibody, same conditions. Images courtesy of Dr. Javier Casas at The Scripps Research Institute.



Description:

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Other Names: T8, Lyt2, Ly-2
Structure: Ig superfamily, CD8α chain, 34 kD
Distribution: Most thymocytes, T cell subset, some NK cells
Function: Co-receptor for TCR
Ligand Receptor: MHC class I molecule
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.