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Safety Data Sheet (SDS) Purified Anti-rat CD8b Antibody     Product Data Sheet (PDF)    
Purified Anti-rat CD8b Antibody
1603510 500 µg $150.00       
Clone: 341
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: CD8+ Wistar rat splenic T cell hybridomas
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IP, WB, IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, immunohistochemical staining1,2 of acetone-fixed frozen sections and Zinc-fixed paraffin-embeded sections, in vitro blocking of allogeneic responses1, Macrophage stimulation3,4 and in vivo depletion.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. (IP IHC WB)
2. Kuhnlein P, et al. 1994. J. Immunol. 153:979. (IHC)
3. Hirji N, et al. 1997. J. Immunol. 158:1833.
4. Hirji N, et al. 1998. J. Immunol. 160:6004.

LOU rat splenocytes stained with

LOU rat splenocytes stained with purified 341, followed by anti-mouse IgG FITC



Description:

CD8b antigen is also known as T8, Lyt2, Ly-2, and CD8β. It is a member of the immunoglobulin superfamily expressed on most thymocytes, some mature T cell subsets, and macrophages. CD8b forms heterodimers with the CD8α chain (CD8a). CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).

Other Names: CD8, Lyt3
Structure: Ig superfamily, CD8β chain
Distribution: Most thymocytes, subset of mature T cells (i.e. including most T suppressor/cytotoxic cells), macrophages
Function: Co-receptor for TCR
Ligand Receptor: MHC class I molecule
Antigen References:

1. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841.
2. Kuhnlein P, et al. 1994. J. Immunol. 153:979.
3. Hirji N, et al. 1997. J. Immunol. 158:1833.
4. Hirji N, et al. 1998. J. Immunol. 160:6004.