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Safety Data Sheet (SDS) Biotin Anti-mouse CD278 ICOS Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse CD278 (ICOS) Antibody
1187015 50 µg $85.00       
Clone: 7E.17G9
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Mouse ICOS cDNA and ICOS hexahistidine fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: blocking of ligand binding.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume.

Application References:

1. Akbari O, et al. 2002. Nat. Med. 8:1024.
2. Harada H, et al. 2003. J. Clin. Invest. 112:234.
3. McAdam AJ, et al. 2000. J. Immunol. 165:5035. (FC Block)
4. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed

Con A-stimulated (3 days) Balb/c

Con A-stimulated (3 days) Balb/c splenocytes stained with biotinylated 7E.17G9, followed by Sav-PE



Description:

The 7E.17G9 antibody reacts with the 47-57 kD ICOS protein, also known as inducible costimulatory molecule, and H4. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes and can costimulate T cells and induce proliferation. In addition ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand, B7h/B7RP-1 and B7-H2 is constitutively expressed in B cell areas of secondary lymphoid organs and can be induced in other tissues by LPS. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. ICOS stimulation has been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.

Other Names: Inducible COStimulatory molecule, H4
Structure: CD28/CTLA-4, 47-57kD
Distribution: Activated T cells, subset of thymocytes
Function: Costimulates T cell activation, proliferation, humoral immune response
Ligand Receptor: B7h/B7RP-1/GL-50
Antigen References:

1. Rudd CE, et al. 2003. Nat. Rev. Immunol. 3:544.
2. McAdam AJ, et al. 2000. J. Immunol. 165:5035.
3. Mak TW, et al. 2003. Nat. Immunol. 4:765