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Safety Data Sheet (SDS) FITC Anti-mouse H-2Kk Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse H-2Kk Antibody
1181520 500 µg $245.00       
Clone: AF3-12.1
Isotype: Mouse (BALB/c) IgG1, κ
Reactivity: Mouse k haplotype, Does not react with other haplotypes (e.g. b, d, f, j, p, q, r, s, u, v)
Immunogen: A/J mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Wasowska B, et al. 2001. Transplantation 71:727.
2. Yamamoto M, et al. 1998. J. Immunol. 160:2188.

C3H mouse splenocytes stained with

C3H mouse splenocytes stained with AF3-12.1 FITC



Description:

The AF3-12.1 antibody reacts with the H-2Kk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kk haplotype. H-2Kk is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF3-12.1 antibody does not cross-react with other haplotypes (b, d, f, j, p, q, r, s, u, v).

Other Names: MHC class I
Structure: MHC class I
Distribution: Nucleated cells of the H-2kk haplotype
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD8
Antigen References:

1. Watts C. 1997. Ann. Review Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323.
3. Ian A, et al. 1996. Ann. Rev. Immunol. 14:369.