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Safety Data Sheet (SDS) Biotin Anti-mouse I-Ab Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse I-Ab Antibody
1182015 50 µg $75.00       
1182020 500 µg $225.00       
Clone: AF6-120.1
Isotype: Mouse (BALB/c) IgG2a, κ
Reactivity: Mouse b haplotype, Cross-reacts with H-2 k and H-2 u haplotypes. Does not react with other haplotypes (e.g., d, f, q, r, s).
Immunogen: C57BL/10J splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Application Notes:

 Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed5; OCT-embedded, ethanol-fixed sections7), immunofluorescence microscopy3 (including acetone-fixed epidermal sheets6), immunoprecipitation7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5).

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Wall KA, et al. 1983. J. Immunol. 131:1056. (FC)
2. Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. (FC)
3. Inaba K, et al. 1998. J. Exp. Med. 188:2163 (IF)
4. Hamrah P, et al. 2002. Invest Opthalmol Vis. Sci. 43:639 (IF)
5. Buono C, et al. 2003. Arterioscler. Thromb. Vasc. Biol. 23:454. (IHC)
6. Wang Z, et al. 2004. J. Immunol. 172:5924. (IHC IF)
7. Nakagawa TY, et al. 1999. Immunity 10:207. (IP)
8. Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC IP) PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with biotinylated AF6-120.1, followed by Sav-PE



Description:

The AF6-120.1 antibody reacts with the I-Ab MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The AF6-120.1 antibody cross-reacts with H-2k and H-2u haplotypes; this antibody does not cross-react with other haplotypes (d, f, q, r, s).

Other Names: MHC class II
Structure: MHC class II
Distribution: B cell and activated T cells, APCs of H-2b mice
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD4
Antigen References:

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.