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Safety Data Sheet (SDS) Biotin Anti-mouse IgMb Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse IgMb Antibody
2631020 500 µg $235.00       
Clone: AF6-78
Isotype: Mouse (BALB/c) IgG1, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested
ELISA Detection - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, depletion of lymphocytes.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References: 1. Stall AM, et al. 1984. J. Immunol. 132:787.
2. Velardi A, et al. 1984. J. Immunol. 133:2098.
C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with biotinylated AF6-78, followed by Sav-PE



Description: This monoclonal antibody was raised by immunization with C57BL/10 mouse splenocytes. It specifically reacts with IgM of Igh-Cb and the related haplotypes (d and n), but not with the free μ heavy chain of IgM. Some cross-reaction with IgM of Igh-Ce can be detected with this antibody.
Other Names: Igh-6b