Sony Biotechnology Inc. Sony Biotechnology Inc.cart
About Us Products What's New Library News Calendar Contact Us Place Order
Safety Data Sheet (SDS) FITC Anti-mouse IgMb Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse IgMb Antibody
2631025 50 µg $95.00       
2631030 500 µg $275.00       
Clone: AF6-78
Isotype: Mouse (BALB/c) IgG1, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, depletion of lymphocytes.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Stall AM, et al. 1984. J. Immunol. 132:787.
2. Velardi A, et al. 1984. J. Immunol. 133:2098.
3. Duong BH, et al. 2010. J. Immunol. 184:4143. PubMed
4. Aschermann S, et al. 2013. PNAS. 110:19042. PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with AF6-78 FITC



Description:

This monoclonal antibody was raised by immunization with C57BL/10 mouse splenocytes. It specifically reacts with IgM of Igh-Cb and the related haplotypes (d and n), but not with the free μ heavy chain of IgM. Some cross-reaction with IgM of Igh-Ce can be detected with this antibody.

Other Names: Igh-6b