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Safety Data Sheet (SDS) Biotin Anti-mouse H-2Kb Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse H-2Kb Antibody
1182515 50 µg $75.00       
1182520 500 µg $225.00       
Clone: AF6-88.5
Isotype: Mouse (BALB/c) IgG2a, κ
Reactivity: Mouse b haplotype, Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v)
Immunogen: C57BL mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Loken MR, et al. 1982. J. Immunol. Methods 50:R85.
2. Wall KA, et al. 1983. J. Immunol. 131:1056.
3. Andersson M, et al. 1998. J. Immunol. 161:6475.
4. Shao H, et al. 2005. J. Immunol. 175:1851.
5. Hui S, et al. 2005. J. Immunol. 175:1851. PubMed
6. Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed
7. Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with biotinylated AF6-88.5, followed by Sav-PE



Description:

The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kb haplotype. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).

Other Names: MHC class I
Structure: MHC class I
Distribution: Nucleated cells of the H-2Kb haplotype
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD8
Antigen References:

1. Watts C. 1997. Ann. Review Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.