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Safety Data Sheet (SDS) Purified Anti-mouse CD122 IL-2Rbeta Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD122 (IL-2Rβ) Antibody
1129510 500 µg $190.00       
Clone: 5H4
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Rat myeloma YB2/0 transfected with truncated mouse Il-2Rβ cDNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IP - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Furse R, et al. 1993 Eur. J. Immunol. 23:3181. (IP)
2. He YW, et al. 1995. J. Immunol. 154:1596. (IP)
3. Liqons DL, et al. 2012. J Biol Chem. 287:34386. PubMed.

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with 5H4 PE and CD3 APC



Description:

CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The 5H4 antibody does not block IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.

Other Names: IL-2 Receptor β chain, IL-2Rβ
Structure: Ig superfamily, forms high affinity IL-2 receptor with CD25 and CD132 chains or intermediate affinity receptor with CD132 alone, 70-75 kD
Distribution: T cells and B cells, NK cells, monocytes, macrophages
Function: Critical component of IL-2 and IL-15 signaling
Ligand Receptor: IL-2, IL-15
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245.
3. Suzuki H, et al. 1995. Science 268:1472.
4. Shi Z, et al. 2009. Eur. J. Immunol. 39:2109.