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Safety Data Sheet (SDS) Biotin Anti-mouse IgMa Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse IgMa Antibody
2643015 50 µg $85.00       
2643020 500 µg $235.00       
Clone: MA-69
Isotype: Mouse (C57BL/6) IgG1, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested
IHC, ELISA Detection - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, stimulation of B cell proliferation, and immunohistochemistry of acetone-fixed frozen sections.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Sieckmann D, et al. 1991. Hybridoma 10:121.
2. Stall AM. 1996. Weir's Handbook of Experimental Immunology pp. 27.

BALB/c splenocytes stained with MA-69

BALB/c splenocytes stained with MA-69 Biotin, followed by Sav-PE



Description:

This antibody was raised by immunization with IgMa mAb TC31. It specifically reacts with mouse IgM of Igh-Ca and the related haplotypes (c, f, g, h, and j). It has been reported that beads coated with MA-69 antibody can stimulate B cell proliferation.

Other Names: Igh-6a