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Safety Data Sheet (SDS) APC Anti-rat CD8a Antibody     Product Data Sheet (PDF)    
APC Anti-rat CD8a Antibody
1603045 25 µg $85.00       
1603050 100 µg $255.00       
Clone: G28
Isotype: Mouse IgG2a, κ
Reactivity: Rat
Immunogen: CD8+ Wistar rat splenic T cell hybridomas
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking1 of allogeneic responses, and immunohistochemical staining of acetone-fixed frozen sections.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References: 1. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841.
2. Matta B, et al. 2008. Am J Pathol. 173:1440. PubMed
LOU rat splenocytes stained with

LOU rat splenocytes stained with G28 APC



Description: CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 αβ heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).
Other Names: CD8a, T8, Leu-8, Ly-2, Lyt2
Structure: Ig superfamily, CD8α chain
Distribution: Most thymocytes, T cell subset; activated CD4+ T cells (not resting), most NK cells and intestinal intraepithelial cells express the CD8α chain without the CD8β chain
Function: Co-receptor for TCR
Ligand Receptor: MHC class I molecule
Antigen References: 1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609.
2. Dallman MJ, et al. 1982. Eur. J. Immunol. 12:511.
3. Cantrell DA, et al. 1982. Immunol. 45:97.
4. Lyscom N, et al. 1982. Immunol. 45:775.
5. Johnson P, et al. 1985. EMBO. J. 4:2539.
6. Barclay AN. 1981. Immunol. 42:593.