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Safety Data Sheet (SDS) APC Anti-rat CD28 Antibody     Product Data Sheet (PDF)    
APC Anti-rat CD28 Antibody
1604540 100 µg $255.00       
Clone: JJ319
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: Rat CD28-transfected BALB/c mouse A2OJ cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The JJ319 antibody has been reported to augment in vitro T cell proliferation and enhance IL-2 production. Additional reported applications (for the relevant formats) include: immunoprecipitation, in vitro costimulation of proliferation responses1, and in vitro costimulation of IL-2 production in the presence of R73 antibody (anti-rat α/β TCR, Cat. No. 201112)2.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Tacke M, et al. 1995. J. Immunol. 154:5121. (Costim)
2. Tacke M, et al. 1997. Eur. J. Immunol. 27:239. (Costim)

LOU rat splenocytes stained with

LOU rat splenocytes stained with JJ319 APC



Description:

CD28 is a 44 kD glycoprotein also known as Tp44 or T44. It is a member of the immunoglobulin superfamily and is expressed on thymocytes, α/βTCR+ T cells, most γ/δTCR+ T cells, and an NK cell subset. During interaction with CD80 (B7-1) or CD86 (B7-2), CD28 acts as the second signal for T cell stimulation. 

Other Names: Tp44, T44
Structure: Ig superfamily, 44 kD
Distribution: All α/βTCR+ T cells, most γ/δTCR+ T cells, NK cell subset
Function: T cell costimulation
Ligand Receptor: CD80 (B7-1) and CD86 (B7-2)
Antigen References:

1. Tacke M, et al. 1995. J. Immunol. 154:5121.
2. Mitnancht R, et al. 1995. Eur. J. Immunol. 25:328.
3. Tacke M, et al. 1997. Eur. J. Immunol. 27:239.