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Safety Data Sheet (SDS) Purified Anti-mouse Ly-51 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse Ly-51 Antibody
1141510 500 µg $190.00       
Clone: 6C3
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: C57BL/6 mouse Pre-B lymphoma cell line (L1-2) plus Abelson murine leukemia virus-specific cytotoxic T-cell clones
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IP, IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2 and immunohistochemical staining3-5, 7-8 of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Pillemer E, et al. 1984. P. Natl. Acad. Sci. USA 81:4434. (IP)
2. Hardy RR, et al. 1991. J. Exp. Med. 173:1213. (IP)
3. Adkins B, et al. 1988. Immunogenetics 27:180. (IHC)
4. Surh CD, et al. 1992. J. Exp. Med. 176:611. (IHC)
5. Goverman J, et al. 1997. Immunol. Today 18:204. (IHC)
6. Dumont-Lagace M, 2014. J. Immunol. 192:2219. PubMed
7. Kvell K, et al. 2010. PLoS One 5:e10701. (FC, IHC)
8. Griewank K, et al. 2007. Immunity 27:751. (FC, IHC)

C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells stained with 6C3 PE and B220 FITC



Description:

Ly-51 is a 140 kD protein also known as 6C3/BP-1. It is a homodimeric cell-surface glycoprotein with aminopeptidase A (APA) activity. Ly-51 is expressed on B cell progenitors, bone marrow stromal cell lines, thymic dendritic cells, and cortical epithelial cells. Ly-51 expression can be upregulated by IL-7 stimulation.

Other Names: 6C3/BP-1
Structure: Aminopeptidase A, 140 kD
Distribution: B progenitors, bone marrow stromal lines, thymic dendritic cells, cortical epithelial cells
Function: Aminopeptidase, may function in IL-7-driven proliferation of B cell precursors
Antigen References:

1. Goverman J, et al. 1997. Immunol. Today 18:204.
2. Morse HC, et al. 1987. J. Exp. Med. 165:920.
3. Sherwood P, et al. 1990. Int. Immunol. 2:399.
4. Lin Q, et al. 1998. J. Immunol. 160:4681.