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Safety Data Sheet (SDS) FITC Anti-mouse I-Ek/rat RT1D Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse I-Ek/rat RT1D Antibody
1151025 50 µg $75.00       
Clone: 14-4-4S
Isotype: Mouse (C3H.SW) IgG2a, κ
Reactivity: Mouse, Cross-reacts with H-2d,p,r haplotypes. Mice of the H-2b,f,q,s haplotypes do not express I-E antigens. Cross-reacts with rat MHC class II antigen RT1D
Immunogen: C3H mouse skin graft and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation2, in vitro induction of B-cell differentiation3, in vitro blocking of antigen presentation4, and immunohistochemical staining5,6 of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Ozato K, et al. 1980. J. Immunol. 124:533.
2. Nei U, et al. 1994. Int. Immunol. 6:61. (IP)
3. Harton JA, et al. 1991. Immunogenetics 34:358.
4. Roy M, et al. 1995. Eur. J. Immunol. 25:596.
5. Farr A, et al. 1996. Eur. J. Immunol. 26:3185. (IHC)
6. Desmet C, et al. 2004. J. Immunol. 173:5766. (IHC)
7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)

C3H/He mouse splenocytes stained with

C3H/He mouse splenocytes stained with 14-4-4S biotin and detected with Sav-PE



Description:

The 14-4-4S antibody reacts with the I-Ek MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 14-4-4S antibody cross-reacts with cells from mice of the H-2d, H-2p, and H-2r haplotypes and the rat MHC class II antigen RT1D. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen. The 14-4-4S antibody has been reported to block antigen presentation and induce differentiation of mouse cells bearing I-Ek.

Other Names: I-Ek, MHC class II
Structure: MHC class II
Distribution: B cell and activated T cells, APCs of H-2b mice
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD4
Antigen References:

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.