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Safety Data Sheet (SDS) APC Anti-rat TCR Alpha/beta Antibody     Product Data Sheet (PDF)    
APC Anti-rat TCR α/β Antibody
1605550 100 µg $255.00       
Clone: R73
Isotype: Mouse IgG1, κ
Reactivity: Rat, Cross-Reactivity: Cynomolgus, Rhesus
Immunogen: Rat T cell blasts & erythrocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, immunohistochemical staining of acetone-fixed frozen sections1, in vitro activation of α/β TCR+ T cells 1,2 and in vivo depletion2 of α/β TCR+ T cells .

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Hunig T, et al. 1989. J. Exp. Med. 169:73.
2. Mitnacht R, et al. 1995. Eur. J. Immunol. 25:328.
3. Brenan M, et al. 1997. Eur. J. Immunol. 27:3070.
4. Yoshino N, et al. 2000. Exp. Anim. 49:97.

LOU rat splenocytes stained with

LOU rat splenocytes stained with R73 APC



Description:

TCR α and β are members of the immunoglobulin superfamily and components of the CD3/TCR complex expressed on most peripheral T cells, intestinal epithelial lymphocytes, and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation. The TCR α/β recognizes peptide bound to MHC class I and II.

Other Names: TCR α/β, α/β TCR
Structure: Ig superfamily, CD3/TCR complex with CD3 and TCR-α subunit, 40 and 46 kD
Distribution: Most peripheral T cells, intestinal epithelial lymphocytes, thymocytes
Function: Antigen recognition, T cell activation/responses
Ligand Receptor: Peptide bound-MHC class I and II
Antigen References:

1. Hunig T, et al. 1989. J. Exp. Med. 169:73.
2. Fangmann J, et al. 1991. Eur. J. Immunol. 21:753.
3. Mitnacht R, et al. 1995. Eur. J. Immunol. 25:328.
4. Peiters RH, et al. 1995. Biochem. Biophys. Res. Commun. 214:552.
5. Brenan M, et al. 1997. Eur. J. Immunol. 27:3070.