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Safety Data Sheet (SDS) Biotin Anti-mouse H-2Kd Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse H-2Kd Antibody
1183015 50 µg $75.00       
1183020 500 µg $235.00       
Clone: SF1-1.1
Isotype: Mouse (SJL) IgG2a, κ
Reactivity: Mouse d haplotype, Weakly cross-reacts with H-2k haplotype, Does not cross react with other haplotypes (e.g., b, j, p, q, s, v).
Immunogen: BALB/c Mouse cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The H-2Kd antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The SF1-1.1 antibody is weakly cross-reactive with H-2k but does not cross-react with other haplotypes (b, j, p, q, s, v). Clone SF1-1.1 recognizes the α3 domain of Kd.

Additional reported applications (for the relevant formats) include: immunoprecipitation1,4 and Western blotting2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Noun G, et al. 1996. J. Immunol. 157:2455. (IP)
2. Abasto JP, et al. 1993. J. Immunol. 151:3569. (WB)
3. Bashuda H, et al. 1997. Transplantation 63:113.
4. Sester M, et al. 2000. J. Biol. Chem. 34:113. (IP)
5. Ma XT, et al. 2006. Cancer Res. 66:1169. (FC)
6. Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC)
7. Norian L, et al. 2004. J. Immunol.. 173:835. PubMed
8. Delon J, et al. 1998. Immunity 9:467.

Balb/c mouse splenocytes stained with

Balb/c mouse splenocytes stained with biotinylated SF1-1.1, followed by Sav-PE



Description:

The SF1-1.1 antibody reacts with the H-2Kd MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kd haplotype. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.

Other Names: MHC class I
Structure: MHC class I
Distribution: Nucleated cells of the H-2Kd haplotype
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD8
Antigen References:

1. Watts C. 1997. Annu. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.
3. York I, et al. 1996. Annu. Rev. Immunol. 14:369.