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Safety Data Sheet (SDS) PE Anti-mouse H-2Kd/H-2Dd Antibody     Product Data Sheet (PDF)    
PE Anti-mouse H-2Kd/H-2Dd Antibody
1173540 200 µg $265.00       
Clone: 34-1-2S
Isotype: Mouse (C3H) IgG2a, κ
Reactivity: Cross-reacts with H-2Kb, s, r, q, p
Immunogen: BDF Splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation2, complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Ozato K, et al. 1982. Transplantation 34:113.
2. Sester M, et al. 2000. J. Biol. Chem. 34:113.

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with 34-1-2S PE

C3H/He mouse splenocytes stained with

C3H/He mouse splenocytes stained with 34-1-2S PE



Description:

The 34-1-2S antibody reacts with the H-2Kb/H-2Dd MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kb/H-2Dd haplotype. H-2Kb/H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-1-2S antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes.

Other Names: MHC class I
Structure: MHC class I
Distribution: Nucleated cells of the H-2Kb/H-2Dd haplotype
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD8
Antigen References:

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York IA, et al. 1996. Ann. Rev. Immunol. 14:369.