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Safety Data Sheet (SDS) Purified Anti-mouse H-2Kk Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse H-2Kk Antibody
1174510 500 µg $210.00       
Clone: 36-7-5
Isotype: Mouse (A.TL) IgG2a, κ
Reactivity: Mouse k haplotype, Weakly cross-reacts with SJL/Hsd mice splenocytes, but does not react with alloantigens of other haplotypes (e.g., b, d, q).
Immunogen: A.AL mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity, and immunohistochemistry2,3 of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Goudeau B, et al. 2003. P. Natl. Acad. Sci. USA 100:15800.
2. Johnson RM. 2004. Infect. Immun. 72:3951.
3. Ardehali A, et al. 1995. Circulation 92:450.

C3H/He mouse splenocytes stained with

C3H/He mouse splenocytes stained with 36-7-5 PE

BALB/c mouse splenocytes stained with

BALB/c mouse splenocytes stained with 36-7-5 PE



Description:

The 36-7-5 antibody reacts with the H-2Kk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kk haplotype. H-2Kk is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 36-7-5 antibody does not react with alloantigens form other haplotypes (b, d, q), but weakly cross-reacts with splenocytes from SJL/Hsd mice.

Other Names: MHC class I
Structure: MHC class I
Distribution: Nucleated cells of the H-2kk haplotype
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD8
Antigen References:

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. Ian A, et al. 1996. Ann. Rev. Immunol. 14:369.