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Safety Data Sheet (SDS) PE Anti-mouse CD49d Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD49d Antibody
1118525 50 µg $100.00       
1118530 200 µg $245.00       
Clone: 9C10 (MFR4.B)
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: (C57BL/6 x A/J)F1 mouse fetal liver mas
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vitro blocking of cell-cell adhesion and T cell costimulation1,2, and immunohistochemistry3 of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
2. Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block)
3. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
4. Jia W, et al. 2005. Blood 106:3854. (FC)
5. Simonetti G, et al. 2013. J Exp Med. 210:2887. PubMed
6. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
7. Di C, et al. 2015. J Biol Chem. 290:12523. PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with 9C10 PE



Description:

CD49d is a 150 kD glycoprotein, also known as α4 integrin or VLA-4 α chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells. CD49d is a heterodimer expressed with either of two β chains, β1 (CD29) or β7, to form the VLA-4 (integrin α4β1) or LPAM-1 (integrin α4β7) complexes. CD49d plays a critical role in both adhesion and T cell costimulation. The primary ligands for CD49d are VCAM-1, MAdCAM-1, and fibronectin. The 9C10(MFR4.B) antibody, in combination with the R1-2 monoclonal antibody, completely blocks VCAM-1 binding to VLA-4.

Other Names: α4 integrin, VLA-4 α chain, integrin α4, ITGA4
Structure: Integrin family, associates with integrin β1 or β7, 150 kD
Distribution: T cells and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, dendritic cells
Function: T cell costimulation, cell-cell and cell-matrix adhesion
Ligand Receptor: VCAM-1, MAdCAM-1, fibronectin
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lobb RR, et al. 1994. J. Clin. Invest. 94:1722.
3. Berlin C, et al. 1993. Cell 74:185.
4. Maguire JE, et al. 1995. J. Exp. Med. 182:2079.