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Safety Data Sheet (SDS) Biotin Anti-mouse CD19 Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse CD19 Antibody
1107520 500 µg $225.00       
Clone: MB19-1
Isotype: Mouse IgA, κ
Reactivity: Mouse
Immunogen: mouse CD19+ pre-B cell line 300.19
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation3, and in vitro costimulation (synergistic with anti-IgM) of B cell activation1,2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Sato S, et al. 1997. J. Immunol. 158:4662. (Costim)
2. Sato S, et al. 1997. P. Natl. Acad. Sci. USA 94:13158. (Costim)
3. Krop I, et al. 1996. Eur. J. Immunol. 26:238. (IP)
4. Sato S, et al. 1996. J. Immunol. 157:4371.
5. Stolk M, et al. 2006. J. Leukoc. Biol. doi:10.1189/jlb.1205739. (FC)
6. Shime H, et al. 2012. PNAS. 109:2066. PubMed.

C57BL/6 splenocytes stained with MB19-1

C57BL/6 splenocytes stained with MB19-1 biotin, then detected with Sav-PE



Description:

CD19 is a 95 kD glycoprotein, also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81 forms a molecular complex integral to B cell activation.

Other Names: B4
Structure: Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells
Function: Modulates B cell activation and differentiation
Ligand Receptor: CD21, CD81, Leu-13
Antigen References:

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF,et al. 1994. Immunol. Today 15:437.