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Safety Data Sheet (SDS) PE Anti-mouse / Rat CD29 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse / Rat CD29 Antibody
1111035 50 µg $100.00       
1111040 200 µg $285.00       
Clone: HMβ1-1
Isotype: Armenian Hamster IgG
Reactivity: Mouse, Rat
Immunogen: Purified mouse VLA-4 (α4β1, CD49d/CD29)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry4 of acetone-fixed frozen sections, in vitro blocking of the adhesion of mouse tumor cell lines to extracellular matrix proteins and in vitro inhibition of T cell proliferative responses1, and in vivo inhibition of neutrophil migration2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Noto K, et al. 1995. Int. Immunol. 7:835.
2. Ridger VC, et al. 2001. J. Immunol. 166:3484.
3. Jia W, et al. 2005. Blood 106:3854. PubMed
4. Economopoulou M, et al. 2005. Blood 106:3831.
5. Lawson BR, et al. 2007. J. Immunol. 178:5366.
6. Eisenmann KM, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M703243200. PubMed
7. Hayashi Y, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 294:G778. PubMed
8. Kim DT, et al. 2008. Blood 111:2929. PubMed
9. Hayashi Y, et al. 2008. J Pharmacol Exp Ther. 326:523. PubMed
10. Carlson TR, et al. 2008. Development. 135:2193. PubMed
11. Sangaletti S, et al. 2008. Cancer Res. 68:9050. (Block) PubMed
12. Zuba-Surma EK, et al. 2008. J Mol Cell Cardiol. 44:865. PubMed
13. Zheng Z, et al. 2012. Biochem Biophys Res Commun. 428:309. PubMed
14. Diaferia GR, et al. 2013. Development. 140:3360. PubMed
15. Liang CZ, et al. 2013. Acta Biomater. 9:9423. PubMed
16. Simonetti G, et al. 2013. J Exp Med. 210:2287. PubMed
17. Weckbach LT, et al. 2014. Blood. 123:1887. PubMed
18. Toda S, et al. 2014. Blood. 123:3963. PubMed

Lou rat bone marrow cells

Lou rat bone marrow cells stained with HMβ1-1 biotin, then detected with Sav-PE

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with HMβ1-1 PE



Description:

CD29 is a 130 kD protein, also known as integrin β1, VLA-β chain, or GPIIa. It is a member of the integrin family, expressed broadly on leukocytes, endothelial cells, smooth muscle, and epithelial cells. In association with CD49a-f, CD29 forms the VLA-1 through VLA-6 complexes, respectively. It plays an important role in cell-cell or cell-matrix interaction. The HMß1-1 antibody reacts with both mouse and rat CD29. It is able to block cell adhesion and inhibit T cell proliferation.

Other Names: integrin β1, VLA-β chain, β1 integrin, GPIIa, ITGB1
Structure: Integrin family, 130 kD
Distribution: Leukocytes, endothelial cells, smooth muscle, epithelial cells
Function: Adhesion
Ligand Receptor: Extracellular matrix
Antigen References:

1. Noto K, et al. 1995. Int. Immunol. 7:835.