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Safety Data Sheet (SDS) Purified Anti-mouse TLR4 CD284/MD2 Complex Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse TLR4 (CD284)/MD2 Complex Antibody
1188005 50 µg $100.00       
1188010 200 µg $195.00       
Clone: MTS510
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Ba/F3 cell line transfected with mouse TLR4 /MD2 complex
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IHC, WB - Validated
IF, IP - Reported in the literature

Application Notes:

Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.8 Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.8 In addition to flow cytometric uses, the MTS510 antibody has also been used for immunoprecipitation, immunofluorescence, and blocking of LPS-induced stimulation.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Akashi S, et al. 2000. J. Immunol. 164:3471. (FC, ICFC, IP, Block)
2. Kawasaki K, et al. 2003. J. Immunol. 170:413. (FC)
3. Nalbandian G, et al. 2005. J. Immunol. 175:2666. (FC) PubMed
4. Kanada S, et al. 2011. Blood 117:2211. PubMed
5. Laplate P, et al. 2011. J. Biol Chem. 286:42494. PubMed
6. Khan MA, et al. 2006. Infect. Immun. 74:2522. (IF)
7. Tanigawa T, et al. 2013. Innate Immun. 19:115. PubMed
8. Akashi S, et al. 2003. J. Exp. Med. 198:1035.

Mouse TLR4/MD2 transfected cells were

Mouse TLR4/MD2 transfected cells were stained with purified CD284 (clone MTS510) (filled histogram), or purified rat IgG2a, κ (open histogram), followed by anti-rat IgG FITC.

FFPE mouse gut was stained

FFPE mouse gut was stained with Purified Anti-Mouse TLR4/MD2 Complex (Cat# 117602) or Purified Rat IgG2a, κ at 1:50 (10 µg/mL) using 10 mM Sodium Citrate Buffer pH 6.0 for antigen retrieval and ABC detection method. Cellular localization: Cell Membrane.
Top: Positive Ctrl: FFPE Mouse Gut, 20X Magnification; Bottom: Negative Ctrl: FFPE Mouse Gut, 20X Magnification





Description:

Toll-like receptors are highly conserved from Drosophila to humans and share structural and functional similarities. They are type I transmembrane signaling receptors which activate the innate immune system in response to pathogen invasion. So far, at least 13 TLR members have been identified. The secretory protein, MD2, associates with cell-surface bound TLR4 (CD284) on monocytes, B cells and T cells. TLR4 has been recognized as critical for host recognition of bacterial LPS in conjunction with MD2.

Other Names: Toll-like Receptor 4/MD2 complex, TLR4-MD2-complex, TLR4/MD2, CD284/MD2
Structure: Type I transmembrane protein complex
Distribution: Monocytes, B cells and T cells
Function: Activates the innate immune system against Gram-negative bacterial pathogens by recognition of LPS
Ligand Receptor: Bacterial LPS
Antigen References:

1. Frleta D, et al. 2003. J. Leukocyte Biol. 74:1064.