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Safety Data Sheet (SDS) PE Anti-mouse CD180 RP105 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD180 (RP105) Antibody
1188530 200 µg $310.00       
Clone: RP/14
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: BALB/c mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, B cell activation1,4,5, protection of B cells from apoptosis induced by radiation or dexamethasone and induction of Fas-independent apoptosis1,5.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Miyake K, et al. 1994. J. Exp. Med. 180:1217. (FC IP Activ Apop)
2. Miyake K, et al. 1995. J. Immunol. 154:3333. (IP)
3. Miyake K, et al. 1998. J. Immunol. 161:1348. (IP)
4. Chan VWF, et al. 1998. J. Exp. Med. 188:93. (Activ)
5. Yamashita Y, et al. 1996. J. Exp. Med. 184:113. (Activ Apop)
6. Fujita K, et al. 2012. Immunol Lett. 144:1. PubMed.

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with RP/14 PE



Description:

CD180 is a 105 kD type I membrane protein, also known as RP105 or Ly64. It is a member of the toll-like receptor family consisting of an extracellular leucine-rich repeat (LRR) and a short cytoplasmic tail. It is expressed on most B cells, monocytes/macrophages, and dendritic cells. CD180 associates with MD-1 to form the RP105/MD-1 surface receptor. CD180/MD-1, by working in concert with TLR4, controls B cell recognition and signalling of bacterial LPS. The ligation of RP105 by RP/14 leads to a strong B cell proliferative response, as well as protection from apoptosis induced by radiation or dexamethasone. Interestingly, in some systems, ligation of RP105 leads to Fas-independent apoptosis.

Other Names: CD180, Radio-protective 105-kD protein, RP105
Structure: Member of the toll-like receptor family, 105 kD
Distribution: B cells, monocytes/macrophages, and dendritic cells
Function: Signaling protein involved in innate immunity
Ligand Receptor: LPS
Antigen References:

1. Miyake K, et al. 1994. J. Exp. Med. 180 :1217.
2. Miyake K, et al. 1995. J. Immunol.. 154:3333.
3. Miyake K, et al. 1998. J. Immunol.. 161:1348.
4. Chan VWF, et al. 1998. J. Exp. Med. 188 :93.
5. Yamashita Y, et al. 1996. J. Exp. Med. 184:113.