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Safety Data Sheet (SDS) PE Anti-mouse MD-1 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse MD-1 Antibody
1189025 50 µg $170.00       
Clone: MD-113
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Mouse RP105/MD-1 transfected NRK cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: blocking of LPS-induced B cell proliferation1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Nagai Y, et al. 2002. Blood 99 :1699.

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with MD-113 PE



Description:

MD-1 is a small 28-kD molecule that is usually found in association with CD180 (RP105), a member of the Toll-like receptor family. MD-1 is primarily expressed by B cells, dendritic cells and monocytes, and promotes the recognition of, and subsequent signaling by, LPS in the innate immune system. The MD-113 antibody is reported to be antagonistic for LPS-induced B cell proliferation.

Other Names: MD1
Structure: Acts in conjunction with the toll-like receptor family members, 28 kD
Distribution: B cells, dendritic cells, and monocytes/macrophages
Function: Recognition and signaling in response to LPS
Ligand Receptor: LPS
Antigen References:

1. Nagai Y, et al. 2002. Blood 99 :1699.