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Safety Data Sheet (SDS) Purified Anti-mouse IgM Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse IgM Antibody
2632505 50 µg $60.00       
2632510 500 µg $130.00       
Clone: RMM-1
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The IgM antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
ELISA - Reported in the literature

Application Notes:

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed

C57BL/6 Mouse splenocytes stained with

C57BL/6 Mouse splenocytes stained with purified RMM-1, followed by anti-rat IgG FITC



Description:

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.

Other Names: Immunoglobulin M
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis