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Safety Data Sheet (SDS) FITC Anti-mouse IgM Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse IgM Antibody
2632525 50 µg $75.00       
2632530 500 µg $165.00       
Clone: RMM-1
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
2. Wong TW, et al. 2014. J. Immunol. 192:3548. PubMed
3. Reed SM, et al. 2014. PLoS One. 9:112126. PubMed

C57BL/6 splenocytes stained with RMM-1

C57BL/6 splenocytes stained with RMM-1 FITC



Description:

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.

Other Names: Immunoglobulin M
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis