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Safety Data Sheet (SDS) Purified Anti-human/mouse ZAP-70 Antibody     Product Data Sheet (PDF)    
Purified Anti-human/mouse ZAP-70 Antibody
2167010 100 µg $150.00       
Clone: 1E7.2
Isotype: Mouse IgG1
Reactivity: Human, Mouse
Immunogen: GST-fusion protein corresponding to residues 282-307 of human ZAP-70
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration 0.2 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

ICFC, WB - Quality tested
IP - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation2, Western blotting2.

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis or Western blotting. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. For Western blotting, the suggested working dilution(s)is 0.5-1 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Qian D, et al. 1997. J. Exp. Med. 185:1253. (ICFC)
2. Crespo M, et al. 2003. New Engl. J. Med. 348:1764. (WB IP)
3. Jamroziak K, et al. 2009. Cancer Epidemiol Biomarkers Prev. PubMed
4. Tai TS, et al. 2013. J. Immunol. 190:428. PubMed.

Jurkat cell extract was resolved

Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-ZAP-70 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.



Description:

ZAP-70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP-70 have been linked to selective T cell defects. The ZAP-70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP-70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL). The 1E7.2 monoclonal antibody recognizes human and mouse ZAP-70 and has been shown to be useful for flow cytometry, Western blotting, and immunoprecipitation.


This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.

Other Names: Zeta chain associated protein kinase, SYK related tyrosine kinase
Structure: Protein tyrosine kinase, contains two SH2 domains, 70 kD
Distribution: T cells, NK cells, B-cell chronic lymphocytic leukemia (B-CLL)
Function: Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated. Defects in ZAP-70 causes selective T cell defect
Ligand Receptor: Binds to CD3 zeta chain, also associates with a variety of proteins including SLA, Fyn, RasGap, Lck, Vav1, and Shc
Modification: Multiple phosphorylation sites (Tyr319 phosphorylation essential for full activity)
Antigen References:

1. Chan AC, et al. 1992. Cell 71:649.
2. Chan AC, et al. 1994. Science 264:1559.
3. Arpaia E, et al. 1994. Cell 76:947.