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Safety Data Sheet (SDS) FITC Anti-rat CD2 Antibody     Product Data Sheet (PDF)    
FITC Anti-rat CD2 Antibody
1606515 100 µg $125.00       
Clone: OX-34
Isotype: Mouse IgG2a, κ
Reactivity: Rat
Immunogen: Rat T cell blasts from mixed lymphocyte reactions
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.5% BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemistry1 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections, immunoprecipitation2, partial in vitro blocking of T cell responses3, and in vivo depletion of T cells3.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Williams AF, et al.. 1987. J. Exp. Med. 165:368.
2. Jefferies WA, et al.. 1985. J. Exp. Med. 162:117.
3. Hirahara H, et al.. 1995. Transplantation 59:85.

LOU rat splenocytes stained with

LOU rat splenocytes stained with OX-34 FITC



Description:

CD2, also known as LFA-2, is a member of the immunoglobulin gene family with an approximate molecular weight of 45 kD. It is expressed on thymocytes, peripheral T cells, dendritic epidermal T cells, macrophages in the spleen, and NK cells. CD2 can associate with the T cell receptor and participate in T cell activation through adhesion and signal transduction. CD2 binds to CD48, CD58, and CD59. The OX-34 antibody blocks CD48 binding to CD2. It has been reported to partially block T cell activation and inhibit the mixed lymphocyte reaction in vitro. The OX-34 antibody has also been used to deplete peripheral T cells and prevent allograft rejection in vivo.

Other Names: LFA-2, T11
Structure: Ig superfamily, approximately 45 kD
Distribution: Thymocytes, T cells, dendritic epidermal T cells, splenic macrophages, NK cells
Function: Adhesion, T cell activation
Ligand Receptor: CD48, CD59
Antigen References:

1. Jefferies WA, et al.. 1985. J. Exp. Med. 162:117.
2. Williams AF, et al.. 1987. J. Exp. Med. 165:368.