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Safety Data Sheet (SDS) FITC Anti-rat CD3 Antibody     Product Data Sheet (PDF)    
FITC Anti-rat CD3 Antibody
1607015 100 µg $125.00       
Clone: 1F4
Isotype: Mouse IgM, κ
Reactivity: Rat
Immunogen: F344 rat spleen cells stimulated with PMA and calcium ionophore
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was conjugated with FITC under optimal conditions, and is at >85% purity. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Immobilized 1F4 antibody can induce T cell proliferation in vitro. Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone-fixed frozen sections1 and formaldehyde- fixed paraffin embedded sections4,5 immunofluorescence microscopy3, in vivo activation of T cell responses1, and in vivo inhibition of T cell responses2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Tanaka T, et al. 1989. J. Immunol. 142:2791. (Activ, IHC, IP)
2. Nicholls MR, et al. 1993. Transplantation 55:459. (Block)
3. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74. (IF)
4. Baba T, et al. 2006. Blood 107:2004. (IHC)
5. Fujishiro J, et al. 2010. Am. J. Transplant. 10:1545-55. (IHC-P)
6. Li X, et al. 2009. J. Immunol. 183:3955. (FC) PubMed

LOU rat splenocytes stained with

LOU rat splenocytes stained with 1F4 FITC



Description:

CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation. 

Other Names: T cell antigen receptor complex, T3
Structure: Ig superfamily, approximately 20-25 kD
Distribution: Thymocytes, peripheral T cells, dendritic epidermal T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand Receptor: Peptide antigen/MHC complex
Antigen References:

1. Tanaka T, et al. 1989 J. Immunol. 142:2791.
2. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74.