The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistryof acetone-fixed frozen sections1,2, inhibition of IL-2 production3, inhibition of MBP-induced T cell activation in EAE transfer model3.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC) 2. Shioji K, et al. 2001. Circulation Res. 89:540. (IHC) 3. Mannie MD, et al. 1993. J. Immunol. 151:7293. 4. Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed
LOU rat splenocytes stained with W3/25 FITC
CD4 is a 55 kD glycoprotein also known as T4. Rat CD4 is a member of the immunoglobulin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation.
T4, L3T4, W3/25
Ig superfamily, 55 kD
Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)
T cell co-receptor, T cell activation
MHC class II molecule
1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609. 2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649.
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